599 Introduction to Molecular Genetics

599
Introduction to Molecular Genetics

Some interesting sites on the www for Molecular Genetics:

Genome Databases

Extra Credit Assignment 1 Due April 30 (5 points)

You have cloned a genomic DNA sequence from yeast that complements a mutant yeast strain that has short telomeres (when the plasmid containing this sequence is in the mutant yeast strain the strain has normal telomeres). You sequence the ends of the clone and get the following sequences.
SEQUENCE 1:
ATGGACTATCATCAAGTTTCAATCTGCAAAGAACCACGGTTGAAGAATC
SEQUENCE 2:
AGAAATTCTAATTATCCAATCGAACAAGATCAATTTGAGACCAGCGATT
Use theSaccharomyces cerevisiae genome database to analyze the clone (use BLAST).
Answer the following questions. How big is your clone. Where is it located in the yeast genome? Which gene in this region do you think is complements the telomere phenotype? Is this gene related to any other genes in yeast or other organisms?

791
Molecular Biology I

RAD 3 Sequence

AGGTGTGATCTTGGCAAACTCTACTAGCATAGAACCGTAATTACGAACAATACTAGGA TCGTTTCTGATTTCAAATCTTGAAGAAATTGCNACTTGATCAGAACCCTTGGTAATAAT CATTGGTAGAAATGATTTTTTGGCTAAGGTCATGGCATATGATTTTTGTAAAACAGTTT TAAAGTTTAACATTCTTGGATACATGTCTAATGGTGATTTGGTCCCTGAAGTGATAATA ACGGAAGAAAATCTCTCAAAGACTGGTTTGATGGCAATCGATGCNTCTAAGCAAGTAA ATCTCATAATCGGATTCGGAACTGCACATTTTCGATTTCATACGGTTCAATAATTAGTA AAACCTTCCTCATATNTTGATTAANAATACCAAATTGCATGTCTTCAAGCATAAAATC TCTACTCCGTTACTCNAAANTCN

Ty sequence

tgttggaatagaaatcaactatcatctactaactagtatttacattactagtatattatc atatacggctggcttagaagatgacgcaaatgatgagaaatagtcatctaaattagtgga agctgaaacgcaaggattgataatgtaataggatcaatgaaatataaacatataaaacgg aatgaataatcgtaatattagtatgtagaaatatagattccattttgaggattccta tatccctcgaggagaacttctagtatattctgtatacctaatattatagcctttatcaac aatggaatcccaacaattatctcaacattcacccatttctcatggtagcgcctgtgcttc ggttacttctaaggaagtccacacaaatcaagatccgttagacgtttcagcttccaaaac agaagaatgtgagaaggcttccactaaggctaactctcaacagacaacaacacctgcttc atcagctgtt

Unknown sequence (unique)

1 ATGAGCGCTA TCTATAAATT ATCTATTCAG GGCATACGGT CTTTTGACTC 51 CAATGATAGG GAAACTATTG AATTTGGCAA GCCTCTGACT TTAATAGTCG 101 GCATGAATGG TTCAGGTAAA ACGACTATCA TCGAATGTTT AAAGTACGCT 151 ACCACAGGTG ATCTACCGCC CAACAGCAAG GGAGGAGTAT TCATTCATGA 201 CCCGAAGATA ACTGGTGAAA AGGACATTAG AGCTCAGGTC AAACTGGCGT 251 TTACGAGTGC CAATGGACTC AATATGATTG TCACCAGAAA TATTCAGTTG 301 CTAATGAAAA AGACTACTAC TACATTTAAG ACTTTAGAAG GCCAGTTGGT 351 CGCTATAAAT AATAGTGGTG ACCGCAGTAC TTTGTCCACC CGGTCTCTGG 401 AATTAGACGC ACAAGTACCC CTGTATCTGG GAGTTCCAAA AGCGATCTTG 451 GAATACGTGA TATTCTGTCA CCAAGAAGAC AGCCTGTGGC CACTGAGTGA 501 ACCATCCAAT CTTAAAAAGA AGTTTGATGA AATTTTCCAA GCGATGAAAT 551 TTACAAAGGC TCTAGACAAT TTAAAATCCA TAAAGAAAGA CATGTCCGTG 601 GATATCAAGT TATTAAAGCA ATCAGTAGAA CACTTAAAGT TGGATAAAGA

Unknown sequence (repeated)

CTCTCGGTAGCCAAGTTGGTTTAAGGCGCAAGACTTTAATTTATCACTACGAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGA

599 Introduction to Molecular Genetics

Some interesting sites on the www for Molecular Genetics:

Genome Databases

Human:

Model Organisms:

Other organisms:

Molecular Techniques

For a different look at microorganisms